Quick Answer: How is somatic hybridization carried out?

Somatic hybridization broadly involves in vitro fusion of isolated protoplasts to form a hybrid cell and its subsequent development to form a hybrid plant. … And protoplast fusion is a wonderful approach to overcome sexual incompatibility between different species of plants.

How is somatic hybridization carried out mention one example of a somatic hybrid?

The process of fusion of protoplast of somatic cells derived from different varieties and or species of plants on a suitable nutrient medium to produce the somatic hybrids is called somatic hybridization. For example pomato is the somatic hybrid obtained by the protoplast fusion of tomato and potato.

How are somatic hybrids obtained?

The somatic fusion process occurs in four steps: The removal of the cell wall of one cell of each type of plant using cellulase enzyme to produce a somatic cell called a protoplast. … The cells are then grown into calluses which then are further grown to plantlets and finally to a full plant, known as a somatic hybrid.

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What is somatic hybridization technique?

Somatic hybridization is a technique which allows the manipulation of cellular genomes by protoplast fusion. Its major contribution to plant breeding is in overcoming common crossing barriers among plant species and in organelle genetics and breeding.

What is somatic hybridization and its application?

Protoplast also known as a naked plant cell refers to all the components of plant cell excluding the cell wall. Development of hybrid plants through the fusion of somatic protoplasts of two different plant species/varieties is called somatic hybridization, and such hybrids are known as somatic hybrids.

What is somatic hybridisation give an example?

Answer: The process of fusion of protoplast of somatic cells derived from different varieties and or species of plants on a suitable nutrient medium to produce the somatic hybrids is called somatic hybridization. For example pomato is the somatic hybrid obtained by the protoplast fusion of tomato and potato.

Which of the following is an example of somatic hybridisation?

The intergeneric somatic hybrids are Pomato (Potato x Tomato) and Bomato (Brinjal Tomato).

Which one is required for somatic hybridization?

Regeneration from protoplasts is one prerequisite for the successful use of somatic hybridization. Several attempts have been made to use protoplast fusion among Fagopyrum species, whereas the most successful attempts have been attained in plant regeneration from protoplasts in common buckwheat (F.

What does somatic mean?

1 : of, relating to, or affecting the body especially as distinguished from the germplasm. 2 : of or relating to the wall of the body : parietal.

How is somatic hybridization technique beneficial for farmers?

Somatic hybridization is very useful for plants which is asexual, sterile and that is also beneficial for those plant which has sexual incompatibility with other species. … Also, this method allows cytoplasm transfer between sexually incompatible species.

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What’s a somatic cell?

A somatic cell is any cell of the body except sperm and egg cells. Somatic cells are diploid, meaning that they contain two sets of chromosomes, one inherited from each parent.

What is the use of polyethylene glycol in somatic hybridization?

Abstract. Somatic hybridization in higher plants has come into focus since methods have been established for protoplast fusion and uptake of foreign DNA and organelles by protoplasts. Polyethylene glycol (PEG) was an effective agent for inducing fusion.

Which chemical is used in protoplast fusion?

Several chemicals have been used successfully to induce fusion but the most successful and the one most widely used at the present time is polyethylene glycol (PEG). The effects of PEG are not specific and it will promote the aggregation and fusion of protoplasts from the same or different species.

What is meant by Cybrid?

A cytoplasmic hybrid (or cybrid, a portmanteau of the two words) is a eukaryotic cell line produced by the fusion of a whole cell with a cytoplast. Cytoplasts are enucleated cells. … A cybrid is then a hybrid cell which mixes the nuclear genes from one cell with the mitochondrial genes from another cell.

What is Somaclonal hybridization?

Somatic hybridization broadly involves in vitro fusion of isolated protoplasts to form a hybrid cell and its subsequent development to form a hybrid plant. Plant protoplasts are of immense utility in somatic plant cell genetic manipulations and improvement of crops.

What is somatic hybrid and Cybrid?

In somatic hybrids recom- bination of mitochondrial genome occurs frequently. … Fusion products with the nucleus of one parent and extra-nuclear genome/s of the other parent are referred to as cybrid and the process to obtain cells or plants with such genetic combination/s is called cybridization.

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